IHC
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min;Incubation: Anti-AT2R/AT1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AT2R) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.IF-P
Tissue/cell: rat colon tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37℃ for 20 min;Incubation: Anti-AT2R/AT1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.| Product Name | AT2R Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human AT2R : 151-250/359 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHC |
| IHC:1:100-1:500 IF-P:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AGTR1 |
|---|---|
| Gene Synonyms | AT1 AG2S AT1B AT1R ATR1 AT1AR AT1BR AT2R1 HAT1R AGTR1B |
| Gene Full Name | angiotensin II receptor type 1 |
| Gene Summary | Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2020] |
| Alternative Names | AG2S AGTR1B AT1 AT1AR AT1B AT1BR AT1R AT2R1 ATR1 HAT1R 1810074K20Rik AT1a AT2R1A Agt1ar Agtr-1a Agtr1 Angtr-1a AT2R1B Agtr-1b Angtr-1b AT3 AT1R AGTR1_HUMAN Angiotensin II type-1 receptor (AT1 receptor) AGTR1A AGTRA_MOUSE Angiotensin II type-1 receptor A (AT1 receptor A) AGTRB_MOUSE Angiotensin II type-1 receptor B (AT1 receptor B) AGTRA_RAT AGTRB_RAT angiotensin II receptor type 1 angiotensin receptor 1B angiotensin II type 1 receptor |
| Molecular Weight(MW) | 41 kDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
IHC
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min;Incubation: Anti-AT2R/AT1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AT2R) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IF-P
Tissue/cell: rat colon tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37℃ for 20 min;Incubation: Anti-AT2R/AT1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.| Application Notes | IHC:1:100-1:500 IF-P:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
