WB
Western blot analysis of extracts from HeLa cells using CDT2 Rabbit mAb at 1:1000.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer using CDT2 Rabbit mAb antibody.ICC/IF
Immunofluorescence analysis of HeLa cells labelling CDT2 with CDT2 Rabbit mAb. The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with CDT2 Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Product Name | CDT2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of human CDT2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB: 1:2000-1:20000 IHC: 1:100 ICC/IF: 1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DTL |
|---|---|
| Gene Synonyms | CDT2 RAMP DCAF2 L2DTL |
| Gene Full Name | denticleless E3 ubiquitin protein ligase adapter |
| Gene Summary | Contributes to ubiquitin-protein transferase activity. Involved in several processes, including protein ubiquitination; regulation of G2/M transition of mitotic cell cycle; and translesion synthesis. Located in centrosome; cytosol; and nuclear lumen. Part of Cul4A-RING E3 ubiquitin ligase complex and Cul4B-RING E3 ubiquitin ligase complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | CDT2 RAMP DCAF2 L2DTL |
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Chromosome,Cytoplasm,Cytoskeleton,Membrane,Nucleus |
WB
Western blot analysis of extracts from HeLa cells using CDT2 Rabbit mAb at 1:1000.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer using CDT2 Rabbit mAb antibody.
ICC/IF
Immunofluorescence analysis of HeLa cells labelling CDT2 with CDT2 Rabbit mAb. The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with CDT2 Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Application Notes | WB: 1:2000-1:20000 IHC: 1:100 ICC/IF: 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C.Stable for 12 months from date of receipt. |
| Storage Buffer | Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA |
