IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDT2 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).FC
1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDT2 Recombinant antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | CDT2 Recombinant monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human DTL protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCICC/IF |
| IF/ICC:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DTL |
|---|---|
| Gene Synonyms | CDT2 RAMP DCAF2 L2DTL |
| Gene Full Name | denticleless E3 ubiquitin protein ligase adapter |
| Gene Summary | Contributes to ubiquitin-protein transferase activity. Involved in several processes, including protein ubiquitination; regulation of G2/M transition of mitotic cell cycle; and translesion synthesis. Located in centrosome; cytosol; and nuclear lumen. Part of Cul4A-RING E3 ubiquitin ligase complex and Cul4B-RING E3 ubiquitin ligase complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | DTL 230144C9 CDW1 DCAF2 DDB1- and CUL4-associated factor 2 |
| Molecular Weight(MW) | 79 kD |
| Cellular Localization | Chromosome,Cytoplasm,Cytoskeleton,Membrane,Nucleus |
IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDT2 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
FC
1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDT2 Recombinant antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | IF/ICC:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
