WB
Western blot analysis of Histone H1.3 in C6, 3T3, Hela lysates using Histone H1.3 antibody.ICC/IF
Immunocytochemistry analysis of Histone H1.3(green) in Hela using Histone H1.3 antibody,and DAPI(blue)IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Histone H1.3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.| Product Name | Histone H1.3 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide of human Histone H1.3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:100 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H1-3 |
|---|---|
| Gene Synonyms | H1D H1.3 H1F3 H1s-2 HIST1H1D |
| Gene Full Name | H1.3 linker histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 22 kDa(Observed band size:30 kDa) |
| Cellular Localization | Nucleus, Chromosome. |
| SwissProt ID | P16402 |
|---|
WB
Western blot analysis of Histone H1.3 in C6, 3T3, Hela lysates using Histone H1.3 antibody.
ICC/IF
Immunocytochemistry analysis of Histone H1.3(green) in Hela using Histone H1.3 antibody,and DAPI(blue)
IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Histone H1.3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:100 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
