WB
Western blot analysis of various lysates using Phospho-Histone H1.3-T17/Histone H1.4-T17 Rabbit mAb at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated with Nocodazole (50 ng/mL) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1 s.WB
Western blot analysis of lysates from HeLa cells using Phospho-Histone H1.3-T17/Histone H1.4-T17 Rabbit mAb at 1:2000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated with Nocodazole (100 ng/ml) at 37℃ for 16 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45 s.| Product Name | Phospho-Histone H1.3-T17/Histone H1.4-T17 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | p-T17 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H1-3 |
|---|---|
| Gene Synonyms | H1D H1.3 H1F3 H1s-2 HIST1H1D |
| Gene Full Name | H1.3 linker histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 30 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P16402 |
|---|
WB
Western blot analysis of various lysates using Phospho-Histone H1.3-T17/Histone H1.4-T17 Rabbit mAb at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated with Nocodazole (50 ng/mL) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1 s.
WB
Western blot analysis of lysates from HeLa cells using Phospho-Histone H1.3-T17/Histone H1.4-T17 Rabbit mAb at 1:2000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated with Nocodazole (100 ng/ml) at 37℃ for 16 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45 s.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
