WB
Various lysates were subjected to SDS PAGE followed by western blot with RTN3 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody as loading control.| Product Name | RTN3 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human RTN3 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:5000-1:50000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | RTN3 |
|---|---|
| Gene Synonyms | HAP ASYIP NSPL2 NSPLII RTN3-A1 |
| Gene Full Name | reticulon 3 |
| Gene Summary | This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1 (BACE1), and the production of amyloid-beta. An increase in the expression of any reticulon protein substantially reduces the production of amyloid-beta, suggesting that reticulon proteins are negative modulators of BACE1 in cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, and pseudogenes of this gene are located on chromosomes 4 and 12. [provided by RefSeq, May 2012] |
| Alternative Names | ASYIP HAP NSP like protein 2 NSP like protein II NSPL2 NSPLII reticulon 3 RTN3 RTN3 A1 |
| Molecular Weight(MW) | 113 kDa(Observed band size:25-28 kDa) |
| Cellular Localization | Endoplasmic reticulum membrane,Golgi apparatus membrane |
WB
Various lysates were subjected to SDS PAGE followed by western blot with RTN3 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody as loading control.| Application Notes | WB:1:5000-1:50000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
