WB
Western blot analysis of TRPV1 using anti-TRPV1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRPV1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of TRPV1 using anti-TRPV1 antibody. TRPV1 was detected in an immunocytochemical section of T-47D cells. The section was incubated with rabbit anti-TRPV1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of U87 cells using anti-TRPV1 antibody . Overlay histogram showing U87 cells stained with Rabbit polyclonal antibody to TRPV1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to TRPV1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human TRPV1 recombinant protein (Position: E107-A800). Human TRPV1 shares 92% and 91.1% amino acid (aa) sequence identity with mouse and rat TRPV1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRPV1 |
|---|---|
| Gene Synonyms | VR1 |
| Gene Full Name | transient receptor potential cation channel subfamily V member 1 |
| Gene Summary | Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | Capsaicin receptor DKFZp434K0220 Osm 9 like TRP channel 1 OTRPC1 TRPV1 Vanilloid receptor 1 VR1 |
| Molecular Weight(MW) | 95 kDa |
| Cellular Localization | Cell membrane,Cell projection,Membrane,Postsynaptic cell membrane,Synapse. |
WB
Western blot analysis of TRPV1 using anti-TRPV1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRPV1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of TRPV1 using anti-TRPV1 antibody. TRPV1 was detected in an immunocytochemical section of T-47D cells. The section was incubated with rabbit anti-TRPV1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of U87 cells using anti-TRPV1 antibody . Overlay histogram showing U87 cells stained with Rabbit polyclonal antibody to TRPV1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
