WB
Western blot analysis of Parkin on different lysates with Rat anti-Parkin antibody at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: Parkin Recombinant Antibody - Rat IgG1 (Chimeric), 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rat IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cerebral cortex. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Parkin Recombinant Antibody - Rat IgG1 (Chimeric), 1/500, 1 hour at room temperature. Secondary antibody: 30 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Cerebral cortex. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Parkin Recombinant Antibody - Rat IgG1 (Chimeric), 1/500, 1 hour at room temperature. Secondary antibody: 30 minutes at room temperature.ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Mouse. Sample: Neuro-2a (Mouse brain neuroblastoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: Parkin Recombinant Antibody - Rat IgG1 (Chimeric), 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Rat. Sample: PC-12 (Rat pheochromocytoma cell (undifferentiated)). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: Parkin Recombinant Antibody - Rat IgG1 (Chimeric), 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).| Product Name | ParkinRecombinant Antibody - Rat IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Parkin aa 1-200. |
| Isotype | IgG1 |
|---|---|
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000 IHC:1:500 ICC/IF:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKN |
|---|---|
| Gene Synonyms | PDJ AR-JP LPRS2 PARK2 |
| Gene Full Name | parkin RBR E3 ubiquitin protein ligase |
| Gene Summary | The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq, Jul 2008] |
| Alternative Names | AR JP antibody E3 ubiquitin ligase antibody E3 ubiquitin protein ligase parkin antibody E3 ubiquitin-protein ligase parkin antibody FRA6E antibody LPRS 2 antibody LPRS2 antibody PARK 2 antibody Park2 antibody Parkin 2 antibody Parkinson disease (autosomal recessive juvenile) 2 antibody Parkinson disease (autosomal recessive juvenile) 2 parkin antibody Parkinson disease protein 2 antibody Parkinson juvenile disease protein 2 antibody Parkinson protein 2 E3 ubiquitin protein ligase antibody Parkinson protein 2 E3 ubiquitin protein ligase (parkin) antibody PDJ antibody PRKN 2 antibody PRKN antibody PRKN2 antibody PRKN2_HUMAN antibody Ubiquitin E3 ligase PRKN antibody |
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Mitochondrion, mitochondrion outer membrane, endoplasmic reticulum, cytosol, Nucleus, neuron projection, postsynaptic density, presynapse. |
WB
Western blot analysis of Parkin on different lysates with Rat anti-Parkin antibody at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: Parkin Recombinant Antibody - Rat IgG1 (Chimeric), 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rat IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cerebral cortex. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Parkin Recombinant Antibody - Rat IgG1 (Chimeric), 1/500, 1 hour at room temperature. Secondary antibody: 30 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Cerebral cortex. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Parkin Recombinant Antibody - Rat IgG1 (Chimeric), 1/500, 1 hour at room temperature. Secondary antibody: 30 minutes at room temperature.
ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Mouse. Sample: Neuro-2a (Mouse brain neuroblastoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: Parkin Recombinant Antibody - Rat IgG1 (Chimeric), 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Rat. Sample: PC-12 (Rat pheochromocytoma cell (undifferentiated)). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: Parkin Recombinant Antibody - Rat IgG1 (Chimeric), 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).| Application Notes | WB:1:5000 IHC:1:500 ICC/IF:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
