WB
WB analysis using CHAT Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CHAT antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.WB
WB analysis of PC-12 using CHAT Monoclonal antibody. PC-12 cells were subjected to SDS PAGE followed by western blot with CHAT antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
IHC staining of mouse small intestine using CHAT Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using CHAT antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
IHC staining of rat small intestine using CHAT Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using CHAT antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | CHAT Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CHAT protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:1000-1:4000 IHC:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CHAT |
|---|---|
| Gene Synonyms | CMS6 CMS1A CMS1A2 CHOACTASE |
| Gene Full Name | choline O-acetyltransferase |
| Gene Summary | This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010] |
| Alternative Names | EC:2.3.1.6 Choline O-acetyltransferase choline acetyltransferase Choline acetylase CHOACTase |
| Molecular Weight(MW) | 748 aa, 83 kDa(Observed band size: 67-71 kDa) |
WB
WB analysis using CHAT Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CHAT antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB
WB analysis of PC-12 using CHAT Monoclonal antibody. PC-12 cells were subjected to SDS PAGE followed by western blot with CHAT antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of mouse small intestine using CHAT Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using CHAT antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
IHC staining of rat small intestine using CHAT Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using CHAT antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:1000-1:4000 IHC:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
