WB
Western blot analysis of CaMKII Alpha/CAMK2A using anti-CaMKII Alpha/CAMK2A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CaMKII Alpha/CAMK2A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of CaMKII Alpha/CAMK2A using anti-CaMKII Alpha/CAMK2A antibody. CaMKII Alpha/CAMK2A was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-CaMKII Alpha/CAMK2A Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis using anti- CAMK2A antibody. detected in paraffin-embedded section of rat brain tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and counterstained with DAPI (blue).FC
Flow Cytometry analysis of U87 cells using anti-CaMKII Alpha/CAMK2A antibody. Overlay histogram showing U87 cells stained with Anti-CaMKII Alpha/CAMK2A Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaMKII Alpha/CAMK2A Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to CaMKII Alpha/CAMK2A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human CaMKII alpha/CAMK2A recombinant protein (Position: M1-H478). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAMK2A |
|---|---|
| Gene Synonyms | CAMKA MRD53 MRT63 CaMKIIalpha CaMKIINalpha |
| Gene Full Name | calcium/calmodulin dependent protein kinase II alpha |
| Gene Summary | The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2018] |
| Alternative Names | CaM kinase II subunit alpha CaMK II subunit alpha CAMK2 CAMK2A CAMK2B CAMK2D CAMK2G CAMKA CaMKII alpha KIAA0968 |
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cell projection,Synapse. |
WB
Western blot analysis of CaMKII Alpha/CAMK2A using anti-CaMKII Alpha/CAMK2A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CaMKII Alpha/CAMK2A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of CaMKII Alpha/CAMK2A using anti-CaMKII Alpha/CAMK2A antibody. CaMKII Alpha/CAMK2A was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-CaMKII Alpha/CAMK2A Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis using anti- CAMK2A antibody. detected in paraffin-embedded section of rat brain tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and counterstained with DAPI (blue).
FC
Flow Cytometry analysis of U87 cells using anti-CaMKII Alpha/CAMK2A antibody. Overlay histogram showing U87 cells stained with Anti-CaMKII Alpha/CAMK2A Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaMKII Alpha/CAMK2A Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
