WB
WB analysis of mouse brain using Alpha Synuclein Monoclonal antibody. mouse brain tissue were subjected to SDS PAGE followed by western blot with a-Synuclein Antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.WB
WB analysis of rat brain using Alpha Synuclein Monoclonal antibody. rat brain tissue were subjected to SDS PAGE followed by western blot with a-Synuclein Antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
IHC staining of human brain using Alpha Synuclein Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human brain tissue slide using a-Synuclein antibody at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
IHC staining of mouse brain using Alpha Synuclein Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using alpha-Synuclein antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
IF Staining of mouse cerebellum using Alpha Synuclein Monoclonal antibody. Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Alpha Synuclein antibody at dilution of 1:400 and Multi-rAb Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).FC
FC experiment of SH-SY5Y using Alpha Synuclein Monoclonal antibody. 1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Alpha Synuclein Monoclonal antibody and 488-Conjugated Goat Anti-Mouse IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit.| Product Name | Alpha Synuclein Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human a-Synuclein protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:500-1:2000 IF-P:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SNCA |
|---|---|
| Gene Synonyms | PD1 NACP PARK1 PARK4 |
| Gene Full Name | synuclein alpha |
| Gene Summary | Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016] |
| Alternative Names | 1 a-synuclein SNCA 1B10E9 alpha syn |
| Molecular Weight(MW) | 14 kDa(Observed band size: 18-19 kDa) |
| Cellular Localization | Amyloid,Cell projection,Cytoplasm,Membrane,Nucleus,Secreted,Synapse. |
| SwissProt ID | P37840 |
|---|
WB
WB analysis of mouse brain using Alpha Synuclein Monoclonal antibody. mouse brain tissue were subjected to SDS PAGE followed by western blot with a-Synuclein Antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB
WB analysis of rat brain using Alpha Synuclein Monoclonal antibody. rat brain tissue were subjected to SDS PAGE followed by western blot with a-Synuclein Antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human brain using Alpha Synuclein Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded human brain tissue slide using a-Synuclein antibody at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
IHC staining of mouse brain using Alpha Synuclein Monoclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using alpha-Synuclein antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
IF Staining of mouse cerebellum using Alpha Synuclein Monoclonal antibody. Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Alpha Synuclein antibody at dilution of 1:400 and Multi-rAb Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC
FC experiment of SH-SY5Y using Alpha Synuclein Monoclonal antibody. 1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Alpha Synuclein Monoclonal antibody and 488-Conjugated Goat Anti-Mouse IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit.| Application Notes | WB:1:500-1:2000 IHC:1:500-1:2000 IF-P:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
