WB
Western blot analysis using eNOS antibody against Thp-1(1),Mcf-7(2),Hela(3),Hepg2(4),A549(5),Cos-7(6),K562(7),A431(8) lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-eNOS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | NOS3 |
|---|---|
| Gene Synonyms | 2310005C01Rik 2310065A03Rik bNOS cNOS EC-NOS ECNOS eNOS HEP-NOS i-NOS IHPS1 INOS MAC-NOS MYMY8 N-NOS NC-NOS nNOS NO NOS Nos-1 Nos-2 Nos-3 NOS-I NOS-II NOS2A NOSIII |
| Gene Full Name | nitric oxide synthase 3 |
| Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| Alternative Names | calcium-calmodulin independent nitric oxide synthase iNOS cNOS constitutive endothelial nitric oxide synthase constitutive NOS EC-NOS endothelial nitric oxide synthase endothelial nitric oxide synthase 3 endothelial NOS eNOS hepatocyte NOS inducible nitric oxide synthase inducible NO synthase inducible NOS macrophage NOS N-NOS NC-NOS neuronal nitric oxide synthase neuronal NOS nitric oxidase synthase nitric oxide synthase nitric oxide synthase 1 (neuronal) nitric oxide synthase 1 neuronal nitric oxide synthase 2 inducible nitric oxide synthase 2 inducible macrophage nitric oxide synthase 2A (inducible hepatocytes) nitric oxide synthase 3 (endothelial cell) nitric oxide synthase 3 endothelial cell nitric oxide synthase brain nitric oxide synthase endothelial nitric oxide synthase inducible-like nitric oxide synthase macrophage nitric-oxide synthase nNOS nNOS mu NOS type I NOS type II NOS type III NOS type II Nos3 NOSII NOSIII peptidyl-cysteine S-nitrosylase NOS1 peptidyl-cysteine S-nitrosylase NOS2 |
| Molecular Weight(MW) | 133kDa、68kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Cytoskeleton,Golgi apparatus, Membrane |
| SwissProt ID | P29474 |
|---|
WB
Western blot analysis using eNOS antibody against Thp-1(1),Mcf-7(2),Hela(3),Hepg2(4),A549(5),Cos-7(6),K562(7),A431(8) lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
