WB
Western blot analysis using LC3B antibody against Hela(1),C2C12(2) lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-LC3B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | MAP1LC3B |
|---|---|
| Gene Synonyms | 1010001C15Rik 1010001H21Rik 4922501H04Rik Atg8 ATG8E ATG8F ATG8G LC3 LC3-I LC3-II LC3A LC3B MAP1A/1BLC3 MAP1A/MAP1B MAP1ALC3 MAP1BLC3 Map1lc3 MAP1LC3B-a Mpl3 wu:fb60g11 zbs559 zgc:56434 zgc:77094 |
| Gene Full Name | microtubule associated protein 1 light chain 3 beta |
| Gene Summary | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008] |
| Alternative Names | autophagy-related protein LC3 A autophagy-related protein LC3 B autophagy-related ubiquitin-like modifier LC3 A autophagy-related ubiquitin-like modifier LC3 B LC3 LC3-like protein 1 lc3a lc3b lc3b3 MAP1 light chain 3-like protein 1 MAP1 light chain 3-like protein 2 MAP1A/1B MAP1A/1B LC3 MAP1A/1B light chain 3 A MAP1A/1B light chain 3 B MAP1A/MAP1B LC3 A MAP1A/MAP1B LC3 B MAP1A/MAP1B LC3 B-like MAP1A/MAP1B light chain 3 A MAP1A/MAP1B light chain 3 B MAP1A/MAP1B light chain 3 B-like MAP1A1B-LC3 microtubule-associated protein 1-light chain 3A microtubule-associated proteins 1A/1B light chain 3 microtubule-associated proteins 1A/1B light chain 3 beta 2 microtubule-associated proteins 1A/1B light chain 3A microtubule-associated proteins 1A/1B light chain 3B Microtubule-associated proteins 1A/1B light chain 3B-like protein |
| Molecular Weight(MW) | 15(observed:18kDa) |
| Cellular Localization | Cytoplasm |
| SwissProt ID | Q9GZQ8 |
|---|
WB
Western blot analysis using LC3B antibody against Hela(1),C2C12(2) lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
