ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using NG2/CSPG4 antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5),U251(6),A549(7),Nih3t3(8),Mouse brain tissue(9),Mouse liver tissue(10),Mouse lung tissue(11),Rat testes tissue(12) . 8% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-NG2/CSPG4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CSPG4 |
|---|---|
| Gene Synonyms | 4732461B14Rik AN2 CSPG4A HMW-MAA MCSP MCSPG MEL-CSPG MSK16 NG2 |
| Gene Full Name | chondroitin sulfate proteoglycan 4 |
| Gene Summary | A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008] |
| Alternative Names | AN2 proteoglycan AN2/NG2 proteoglycan cell surface proteoglycan chondroitin sulfate proteoglycan 4 (melanoma-associated) chondroitin sulfate proteoglycan NG2 HMW MAA MEL CSPG melanoma chondroitin sulfate proteoglycan melanoma-associated chondroitin sulfate proteoglycan nerve/glial antigen 2 NG-2 proteoglycan AN2 |
| Molecular Weight(MW) | 250kDa（observed：252kDa) |
| Source | Rabbit |
| Cellular Localization | Cell membrane;Apical cell membrane;Cell projection, lamellipodium membrane;Cell surface |
| SwissProt ID | Q6UVK1 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using NG2/CSPG4 antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5),U251(6),A549(7),Nih3t3(8),Mouse brain tissue(9),Mouse liver tissue(10),Mouse lung tissue(11),Rat testes tissue(12) . 8% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
