IHC
DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30µg/ml Mouse Anti-Human ALPL Antibody Second Ab: 2µg/mL HRPLinked Caprine Anti-Mouse IgG Polyclonal Antibody.IHC
DAB staining on IHC-P; Sample: Human Liver Tissue Primary Ab: 30µg/ml Mouse AntiHuman ALPL Antibody Control: Used PBS instead of primary antibody Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal AntibodyWB
Western Blot; Sample: Lane1: Porcine Liver lysate; Lane2: Porcine Heart lysate; Lane3: Hela cell lysate Primary Ab: 2µg/mL Mouse Anti-Human ALPL Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal AntibodyWB
Western Blot; Samples: Lane1: Porcine Liver lysate; Lane2: Porcine Heart lysate; Lane3: Hela cell lysate; Lane4: LO2 cell lysate; Primary Ab: 1.5µg/ml Mouse AntiHuman ALPL Antibody Second Ab: 0.2?g/ml HRP-Linked 3 / 4 Caprine Anti-Mouse IgG Polyclonal Antibody.| Product Name | Monoclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant ALPL (Leu18~Arg335) expressed in E.coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:0.01-2µg/mL IHC:5-30µg/mL |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | ALPL |
|---|---|
| Gene Synonyms | HOPS HPPA HPPC HPPI HPPO TNAP TNALP APTNAP TNSALP AP-TNAP TNS-ALP |
| Gene Full Name | alkaline phosphatase, biomineralization associated |
| Gene Summary | This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015] |
| Alternative Names | BALP BSAP HOPS AP-TNAP TNSALP Bone Alkaline Phosphatase Alkaline Phosphatase Tissue-Nonspecific Isozyme Alkaline Phosphatase Liver/Bone/Kidney |
IHC
DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30µg/ml Mouse Anti-Human ALPL Antibody Second Ab: 2µg/mL HRPLinked Caprine Anti-Mouse IgG Polyclonal Antibody.
IHC
DAB staining on IHC-P; Sample: Human Liver Tissue Primary Ab: 30µg/ml Mouse AntiHuman ALPL Antibody Control: Used PBS instead of primary antibody Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
WB
Western Blot; Sample: Lane1: Porcine Liver lysate; Lane2: Porcine Heart lysate; Lane3: Hela cell lysate Primary Ab: 2µg/mL Mouse Anti-Human ALPL Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
WB
Western Blot; Samples: Lane1: Porcine Liver lysate; Lane2: Porcine Heart lysate; Lane3: Hela cell lysate; Lane4: LO2 cell lysate; Primary Ab: 1.5µg/ml Mouse AntiHuman ALPL Antibody Second Ab: 0.2?g/ml HRP-Linked 3 / 4 Caprine Anti-Mouse IgG Polyclonal Antibody.| Application Notes | WB:0.01-2µg/mL IHC:5-30µg/mL |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 24 months. |
| Storage Buffer | Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol. |
