WB
Western blot analysis of lysates from Mouse brain, using MAP2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.WB
Western blot analysis of various lysates, using MAP2 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.IF-P
Immunofluorescence analysis of paraffinembedded rat brain using MAP2 Rabbit pAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.IF-P
Immunofluorescence analysis of paraffinembedded mouse brain using MAP2 RabbitpAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat heart using MAP2 Rabbit pAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHCstaining.IHC
Immunohistochemistry analysis of paraffinembedded Human kidney cancer using MAP2Rabbit pAb at dilution of 1:200 (40xlens). Microwave antigen retrieval performedwith 0.01M PBS Buffer (pH 7.2) prior to IHCstaining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain using MAP2 RabbitpAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHCstaining.| Product Name | MAP2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:5000 IF-P:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2 |
|---|---|
| Gene Synonyms | MAP-2 MAP2A MAP2B MAP2C |
| Gene Full Name | microtubule associated protein 2 |
| Gene Summary | This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010] |
| Alternative Names | MAP-2 MAP2A MAP2B MAP2C MAP2 |
| Molecular Weight(MW) | 200kDa(Observed band size:75 kDa/220-280 kDa) |
| Cellular Localization | Cytoplasm, cytoskeleton. |
WB
Western blot analysis of lysates from Mouse brain, using MAP2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
WB
Western blot analysis of various lysates, using MAP2 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
IF-P
Immunofluorescence analysis of paraffinembedded rat brain using MAP2 Rabbit pAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
IF-P
Immunofluorescence analysis of paraffinembedded mouse brain using MAP2 RabbitpAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat heart using MAP2 Rabbit pAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHCstaining.
IHC
Immunohistochemistry analysis of paraffinembedded Human kidney cancer using MAP2Rabbit pAb at dilution of 1:200 (40xlens). Microwave antigen retrieval performedwith 0.01M PBS Buffer (pH 7.2) prior to IHCstaining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain using MAP2 RabbitpAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHCstaining.| Application Notes | WB:1:1000-1:5000 IF-P:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
