WB
Western blot analysis of PAI1 on different lysates with Mouse anti-PAI1 antibody at 1/1000 dilution. Lane 1: no heat NIH/3T3 cell lysate (20 µg/Lane), Lane 2: no heat NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane), Lane 3: NIH/3T3 cell lysate (20 µg/Lane), Lane 4: NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane), Lane 5: HUVEC cell lysate (20 µg/Lane), Lane 6: Mouse placenta tissue lysate (40 µg/Lane), Lane 7: Rat placenta tissue lysate (40 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100000 dilution was used for 1 hour at room temperature.IF-P
Immunofluorescence analysis of paraffin-embedded human placenta tissue labeling PAI1 with Mouse anti-PAI1 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-PAI1 antibody at 1/5000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of HepG2 cells labeling PAI1. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).FC
Flow cytometric analysis of NIH/3T3 cells labeling PAI1. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | PAI1 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human PAI1 aa 31-80 / 402. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCIF-PIHCWB |
| WB:1:1000 IHC:1:5000 FC:1:500-1:1000 IF-P:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SERPINE1 |
|---|---|
| Gene Synonyms | PAI PAI1 PAI-1 PLANH1 |
| Gene Full Name | serpin family E member 1 |
| Gene Summary | This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. The protein also functions as a component of innate antiviral immunity. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. [provided by RefSeq, Aug 2020] |
| Alternative Names | Clade E antibody Endothelial plasminogen activator inhibitor antibody Nexin antibody Nexin plasminogen activator inhibitor type 1 antibody PAI 1 antibody PAI antibody PAI-1 antibody PAI1_HUMAN antibody PLANH1 antibody Plasminogen activator inhibitor 1 antibody Clade E antibody Endothelial plasminogen activator inhibitor antibody Nexin antibody Nexin plasminogen activator inhibitor type 1 antibody PAI 1 antibody PAI antibody PAI-1 antibody PAI1_HUMAN antibody PLANH1 antibody Plasminogen activator inhibitor 1 antibody Plasminogen activator inhibitor type 1 antibody Serine (or cysteine) proteinase inhibitor antibody Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody Serine proteinase inhibitor clade E member 1 antibody serpin antibody Serpin E1 antibody Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody Serpin peptidase inhibitor clade E antibody Serpine 1 antibody SERPINE1 antibody |
| Molecular Weight(MW) | 45kDa(Observed band size:45/100kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of PAI1 on different lysates with Mouse anti-PAI1 antibody at 1/1000 dilution. Lane 1: no heat NIH/3T3 cell lysate (20 µg/Lane), Lane 2: no heat NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane), Lane 3: NIH/3T3 cell lysate (20 µg/Lane), Lane 4: NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane), Lane 5: HUVEC cell lysate (20 µg/Lane), Lane 6: Mouse placenta tissue lysate (40 µg/Lane), Lane 7: Rat placenta tissue lysate (40 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100000 dilution was used for 1 hour at room temperature.
IF-P
Immunofluorescence analysis of paraffin-embedded human placenta tissue labeling PAI1 with Mouse anti-PAI1 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-PAI1 antibody at 1/5000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of HepG2 cells labeling PAI1. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
FC
Flow cytometric analysis of NIH/3T3 cells labeling PAI1. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 IHC:1:5000 FC:1:500-1:1000 IF-P:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
