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Chk2 Antibody

Catalog Number：OM275389

  • Immunofluorescence of Chk2 in Jurkat cells with Chk2 antibody at 5 μg/mL

  • Immunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 μg/mL.

  • Western blot analysis of Chk2 expression in (A) K562, (B) Jurkat, and (C) HL-60 whole cell lysates with Chk2 antibody at 1 μg /ml.

Product Profile

Product Name Chk2 Antibody
Antibody Type Primary Antibodies
Product description 
Chk2 Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.1) Matsuoka S, Huang M, and Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 1998; 282:1893-7.
Immunogen 
Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2. The immunogen is located within the first 50 amino acids of Chk2.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAICCIFWB
Chk2 antibody can be used for detection of Chk2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 5 μg/mL.:
Species Reactivity

HumanMouseRat
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol CHEK2
Alternative Names
Chk2 Antibody: CDS1
CHK2
LFS2
RAD53
hCds1
HuCds1
PP1425
CDS1
CHK2 checkpoint homolog
Hucds1
Molecular Weight(MW) Predicted: 60 kDa Observed: 60 kDa
Sequence Similarities 3) Cat. No. 17-005 - Jurkat Cell Slide

Database Links

Entrez Gene 11200
Protein Accession NP_009125

Application

  • Application

    Immunofluorescence of Chk2 in Jurkat cells with Chk2 antibody at 5 μg/mL

  • Application

    Immunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 μg/mL.

  • Application

    Western blot analysis of Chk2 expression in (A) K562, (B) Jurkat, and (C) HL-60 whole cell lysates with Chk2 antibody at 1 μg /ml.

Positive Control 1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
Chk2 antibody can be used for detection of Chk2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 5 μg/mL.:

Additional Information

Form Liquid
Storage Instructions Chk2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Chk2 Antibody is supplied in PBS containing 0.02% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM275389
  • Price: $336.00
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