Immunofluorescence staining of methanol-fixed HeLa cells using Chk2 (Phospho-Thr383) Antibody.
Western blot analysis of extracts from COS7 cells treated with UV using Chk2 (Phospho-Thr383) Antibody. The lane on the right is treated with the antigen-specific peptide.
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by Chk2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.1) Shannon L. Gibson, Cancer Res., Dec 2005; 65: 10734 - 10741.
Immunogen
Chk2 (phospho Thr383) antibody was raised against a peptide sequence around phosphorylation site of threonine383 (M-R-T (p)-L-C) derived from Human Chk1.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IFWB
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200:
Immunofluorescence staining of methanol-fixed HeLa cells using Chk2 (Phospho-Thr383) Antibody.
Application
Western blot analysis of extracts from COS7 cells treated with UV using Chk2 (Phospho-Thr383) Antibody. The lane on the right is treated with the antigen-specific peptide.
Application Notes
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200:
Additional Information
Form
Liquid
Storage Instructions
Store antibody at -20˚C for up to one year.
Storage Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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