All products Antibodies Cyclin D1 Antibody

Cyclin D1 Antibody

Catalog Number：OM276531

  • Confocal immunofluorescent analysis of Cyclin D1 Antibody (S90) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

  • Western blot analysis of (S90) in mouse lung lysates (35ug/lane).

  • Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 20 ug per lane.

  • Western Blot at 1:2000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

  • Antibody staining Cyclin D1 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Product Profile

Product Name Cyclin D1 Antibody
Antibody Type Primary Antibodies
Product description 
CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. It has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of the gene encoding this protein, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.1) He,Y.Y., Cancer Res. 68 (10), 3752-3758 (2008) Marsit,C.J., Clin. Cancer Res. 14 (8), 2371-2377 (2008) Caldon,C.E., Cancer Res. 68 (8), 3026-3036 (2008)
Immunogen 
This Cyclin D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from human Cyclin D1.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IFIHC-PWB
For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IF starting dilution is: 1:10~50:
Species Reactivity

HumanMouse
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol CCND1
Alternative Names
G1/S-specific cyclin-D1
B-cell lymphoma 1 protein
BCL-1
BCL-1 oncogene
PRAD1 oncogene
CCND1
BCL1
PRAD1
Molecular Weight(MW) 34 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Bovine

Database Links

Entrez Gene 595
Protein Accession P24385

Application

  • Application

    Confocal immunofluorescent analysis of Cyclin D1 Antibody (S90) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

  • Application

    Western blot analysis of (S90) in mouse lung lysates (35ug/lane).

  • Application

    Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 20 ug per lane.

  • Application

    Western Blot at 1:2000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

  • Application

    Antibody staining Cyclin D1 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Application Notes
For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IF starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM276531
  • Price: $336.00
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