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ISWI Antibody

Catalog Number：OM282374

  • Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Western blot analysis in K562 cell line lysates (35ug/lane).

Product Profile

Product Name ISWI Antibody
Antibody Type Primary Antibodies
Product description 
The protein encoded by this gene is a member of the
SWI/SNF family of proteins. Members of this family have helicase
and ATPase activities and are thought to regulate transcription of
certain genes by altering the chromatin structure around those
genes. The protein encoded by this gene is a component of the
chromatin remodeling and spacing factor RSF, a facilitator of the
transcription of class II genes by RNA polymerase II. The encoded
protein is similar in sequence to the Drosophila ISWI chromatin
remodeling protein.1) Goldman, J.A., et al. J. Biol. Chem. 285(7):4645-4651(2010)
Immunogen 
This ISWI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 946-975 amino acids from the C-terminal region of human ISWI.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

FACSWB
For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol SMARCA5
Alternative Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5
364-
Sucrose nonfermenting protein 2 homolog
hSNF2H
SMARCA5
SNF2H
WCRF135
Molecular Weight(MW) 122 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Mouse

Database Links

Entrez Gene 8467
Protein Accession O60264

Application

  • Application

    Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Application

    Western blot analysis in K562 cell line lysates (35ug/lane).

Application Notes
For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM282374
  • Price: $336.00
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