Antibody used in WB on r-SMAD1 at: 1:1000 (Lane 1: 5ug of transfected 293T lysate (SMAD1) , Lane 2: 5ug of transfected 293T lysate (SMAD2) , Lane 3: 5ug of transfected 293T lysate (SMAD3) , Lane 4: 5ug of transfected 293T lysate (SMAD4) , Lane 5: 5ug of transfected 293T lysate (SMAD5) , Lane 6: 5ug of transfected 293T lysate (SMAD6) , Lane 7: 5ug of transfected 293T lysate (SMAD7) , Lane 8: 5ug of
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Product Name | SMAD1 Antibody |
---|---|
Antibody Type | Primary Antibodies |
Product description |
|
Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD1. |
Clonality | Polyclonal |
---|---|
Host Species | Rabbit |
Tested Applications | |
SMAD1 antibody can be used for detection of SMAD1 by ELISA at 1:12500. SMAD1 antibody can be used for detection of SMAD1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
Species Reactivity | |
Concentration | 1 mg/ml |
Purification | Affinity purified |
Gene Symbol | SMAD1 |
---|---|
Alternative Names | SMAD1 BSP1 JV4-1 JV41 MADH1 MADR1 BSP-1 |
Molecular Weight(MW) | 52 kDa |
Entrez Gene | 4086 |
---|---|
Protein Accession | NP_005891 |
Antibody used in WB on r-SMAD1 at: 1:1000 (Lane 1: 5ug of transfected 293T lysate (SMAD1) , Lane 2: 5ug of transfected 293T lysate (SMAD2) , Lane 3: 5ug of transfected 293T lysate (SMAD3) , Lane 4: 5ug of transfected 293T lysate (SMAD4) , Lane 5: 5ug of transfected 293T lysate (SMAD5) , Lane 6: 5ug of transfected 293T lysate (SMAD6) , Lane 7: 5ug of transfected 293T lysate (SMAD7) , Lane 8: 5ug of
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
---|---|
Application Notes | SMAD1 antibody can be used for detection of SMAD1 by ELISA at 1:12500. SMAD1 antibody can be used for detection of SMAD1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
Form | Liquid |
---|---|
Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
2013 © Omnimabs , All Rights Reserved.