All products Antibodies Smad7 Antibody

Smad7 Antibody

Catalog Number：OM291682

  • Fluorescent confocal image of A431 cell stained with Smad7 Antibody . A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Smad7 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).Smad7 immunoreactiv

  • Smad7 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Western blot analysis of Smad7 Antibody in ZR-75-1 cell line lysates (35ug/lane)

Product Profile

Product Name Smad7 Antibody
Antibody Type Primary Antibodies
Product description 
Smad7 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta super family.1) Hirata,H., et.al., Cancer 115 (19), 4488-4503 (2009)
Immunogen 
This Smad7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids from the Central region of human Smad7.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IFIHC-PWB
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml

Target Information

Gene Symbol SMAD7
Alternative Names
Mothers against decapentaplegic homolog 7
MAD homolog 7
Mothers against DPP homolog 7
Mothers against decapentaplegic homolog 8
MAD homolog 8
Mothers against DPP homolog 8
SMAD family member 7
SMAD 7
Smad7
hSMAD7
SMAD7
MADH7
MADH8
Molecular Weight(MW) 46 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Rat

Database Links

Entrez Gene 4092
Protein Accession O15105

Application

  • Application

    Fluorescent confocal image of A431 cell stained with Smad7 Antibody . A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Smad7 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).Smad7 immunoreactiv

  • Application

    Smad7 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Application

    Western blot analysis of Smad7 Antibody in ZR-75-1 cell line lysates (35ug/lane)

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM291682
  • Price: $336.00
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