Application
Western blot analysis of anti-MBD1 Pab in HeLa cell lysate. MBD1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Product Name | MBD1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MBD1; CXXC3; PCM1; Methyl-CpG-binding domain protein 1; CXXC-type zinc finger protein 3; Methyl-CpG-binding protein MBD1; Protein containing methyl-CpG-binding domain 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CXXC3 PCM1 |
|---|---|
| Alternative Names | MBD1 CXXC3 PCM1 Methyl-CpG-binding domain protein 1 CXXC-type zinc finger protein 3 Methyl-CpG-binding protein MBD1 Protein containing methyl-CpG-binding domain 1 |
| Molecular Weight(MW) | 66607 Da |
| Function | Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Nucleus (By similarity). Nucleus matrix (By similarity). Nucleus speckle. Chromosome. Note=Colocalizes with the Ten-1 ICD form of TENM1 in foci associated with the nuclear matrix (By similarity). Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes |
| Entrez Gene | 4152 |
|---|
Application
Western blot analysis of anti-MBD1 Pab in HeLa cell lysate. MBD1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
