Application
Figure 1: Western blot analysis using anti-MCL1 mAb against Hela (1), BCBL-1 (2), Jurkat (3) and HL60 (4) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lymphnode tissues using anti-MCL1 mAb with DAB staining.Application
Figure 3: Confocal immunofluorescence analysis of HepG2 cells using anti-MCL1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Product Name | MCL-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EAT, MCL1L, MCL1S |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8C6, 8C6D4B1 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BCL2L3 |
|---|---|
| Alternative Names | EAT MCL1L MCL1S |
| Molecular Weight(MW) | 37337 Da |
| Function | Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis Isoform 2 promotes apoptosis |
| Tissue Specificity | Purified recombinant fragment of human MCL-1 expressed in E. Coli. |
| Cellular Localization | Membrane; Single-pass membrane protein (Potential). Cytoplasm. Mitochondrion. Nucleus, nucleoplasm Note=Cytoplasmic, associated with mitochondria |
| Entrez Gene | 4170 |
|---|---|
| Nucleotide Accession | NP_877495.1 |
Application
Figure 1: Western blot analysis using anti-MCL1 mAb against Hela (1), BCBL-1 (2), Jurkat (3) and HL60 (4) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lymphnode tissues using anti-MCL1 mAb with DAB staining.
Application
Figure 3: Confocal immunofluorescence analysis of HepG2 cells using anti-MCL1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human MCL-1 expressed in E. Coli. |
