Application
Fluorescent confocal image of SY5Y cells stained with NGFR antibody SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1842a NGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).Application
Western blot analysis of NGFR Antibody (Cat. #AM1842a) in mouse heart tissue lysates (35ug/lane).NGFR (arrow) was detected using the purified Mab.(1:1000)Application
NGFR Antibody(Cat. #AM1842a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NGFR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NGFR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 8G3G10 |
| Host Species | Mouse |
| Tested Applications | IFWBIHC |
| IF:1:100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TNFRSF16 |
|---|---|
| Alternative Names | NGFR TNFRSF16 Tumor necrosis factor receptor superfamily member 16 Gp80-LNGFR Low affinity neurotrophin receptor p75NTR Low-affinity nerve growth factor receptor p75 ICD CD_antigen=CD271 Flags: Precursor |
| Molecular Weight(MW) | 45183 Da |
| Function | Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells |
| Tissue Specificity | This NGFR antibody is generated from mouse immunized with NGFR recombinant protein. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 4804 |
|---|
Application
Fluorescent confocal image of SY5Y cells stained with NGFR antibody SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1842a NGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
Application
Western blot analysis of NGFR Antibody (Cat. #AM1842a) in mouse heart tissue lysates (35ug/lane).NGFR (arrow) was detected using the purified Mab.(1:1000)
Application
NGFR Antibody(Cat. #AM1842a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NGFR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | IF:1:100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
