Application
PAX6 (Cat. #AM1871a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PAX6 antibody detected the PAX6 protein (arrow).Application
Confocal immunofluorescent analysis of PAX6 Antibody (Ascites)(Cat#AM1871a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | PAX6 Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAX6; AN2; Paired box protein Pax-6; Aniridia type II protein; Oculorhombin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 193CT15.2.2 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500~16000 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AN2 |
|---|---|
| Alternative Names | PAX6 AN2 Paired box protein Pax-6 Aniridia type II protein Oculorhombin |
| Molecular Weight(MW) | 46683 Da |
| Function | Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes |
| Tissue Specificity | Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5080 |
|---|---|
| Nucleotide Accession | NP_000271.1 NP_001595.2 |
Application
PAX6 (Cat. #AM1871a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PAX6 antibody detected the PAX6 protein (arrow).
Application
Confocal immunofluorescent analysis of PAX6 Antibody (Ascites)(Cat#AM1871a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:500~16000 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
