Application
Western blot analysis of RNF5 Antibody (N-term) in K562 cell line lysates (35ug/lane). RNF5 (arrow) was detected using the purified Pab.Application
Western blot analysis of RNF5 (arrow) using rabbit polyclonal RNF5 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RNF5 gene.| Product Name | RNF5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF5; G16; NG2; RMA1; E3 ubiquitin-protein ligase RNF5; Protein G16; RING finger protein 5; Ram1 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G16 NG2 RMA1 |
|---|---|
| Alternative Names | RNF5 G16 NG2 RMA1 E3 ubiquitin-protein ligase RNF5 Protein G16 RING finger protein 5 Ram1 homolog |
| Molecular Weight(MW) | 19881 Da |
| Function | Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Membrane; Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane Note=Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles |
| Entrez Gene | 6048 |
|---|
Application
Western blot analysis of RNF5 Antibody (N-term) in K562 cell line lysates (35ug/lane). RNF5 (arrow) was detected using the purified Pab.
Application
Western blot analysis of RNF5 (arrow) using rabbit polyclonal RNF5 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RNF5 gene.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
