Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Adducin alpha (pS59) RabMAb (Cat. #AJ1015c), 1:10,000 dilution. Cells were either (A) untreated (B) treated with calyculin A| Product Name | Adducin alpha Antibody Phospho (pS59) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha |
| Modification | p-S59 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2175AY |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADDA |
|---|---|
| Alternative Names | ADD1 ADDA Alpha-adducin Erythrocyte adducin subunit alpha |
| Molecular Weight(MW) | 80955 Da |
| Function | Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin |
| Tissue Specificity | Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side |
| Entrez Gene | 118 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Adducin alpha (pS59) RabMAb (Cat. #AJ1015c), 1:10,000 dilution. Cells were either (A) untreated (B) treated with calyculin A| Application Notes | WB:1:1000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
