Application
Western blot analysis of ADD1 phosphorylation expression in Forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Product Name | alpha Adducin (phospho Ser726) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alpha Adducin (phospho Ser726)Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide derived from human ADD1 around the phosphorylation site of Ser726 |
| Modification | p-Ser726 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADDA ADD1 (phospho Ser726) Ser726 Phospho ADD1 (phospho Ser726) Phospho Phospho ADD1 (phospho Ser726) adducin 1 (alpha) ADD1 (phospho Ser726) (pS726) Phospho Ser726 ADD1 (phospho Ser726) |
|---|---|
| Target | alpha Adducin (phospho Ser726) |
| Tissue Specificity | ADD1 |
Application
Western blot analysis of ADD1 phosphorylation expression in Forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
