Application
Western blot analysis of extract from HT-29 cells untreated ortreated with Doxorubicin (1mM, 30min), using ADD1 Antibody (S726) (#AE1003a, Lane 1 and 2) and Phospho-ADD1-S726 Antibody (#AE1003b, Lane 3 and 4).Application
Immunohistochemical analysis of paraffin- embeddedhuman lung carcinoma tissue using ADD1 Antibody (S726) (#AE1003a).Application
Immunofluorescence staining of methanol-fixed HeLa cells using ADD1 Antibody (S726) (#AE1003a).| Product Name | ADD1 Antibody (S726) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Erythrocyte adducin subunit alpha; Alpha-adducin |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500~1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADDA |
|---|---|
| Alternative Names | Erythrocyte adducin subunit alpha Alpha-adducin |
| Molecular Weight(MW) | 80955 Da |
| Function | Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin |
| Tissue Specificity | Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side |
| Entrez Gene | 118 |
|---|
Application
Western blot analysis of extract from HT-29 cells untreated ortreated with Doxorubicin (1mM, 30min), using ADD1 Antibody (S726) (#AE1003a, Lane 1 and 2) and Phospho-ADD1-S726 Antibody (#AE1003b, Lane 3 and 4).
Application
Immunohistochemical analysis of paraffin- embeddedhuman lung carcinoma tissue using ADD1 Antibody (S726) (#AE1003a).
Application
Immunofluorescence staining of methanol-fixed HeLa cells using ADD1 Antibody (S726) (#AE1003a).| Application Notes | WB:1:500~1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
