Application
Western blot analysis of KRT8 Antibody (Ascites) (Cat. #AM1827a) in T47D cell line lysates (35ug/lane). KRT8 (arrow) was detected using the Mab ascites (1:1000 dilution).Application
Confocal immunofluorescent analysis of KRT8 Antibody (Ascites)(Cat#AM1827a) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).Application
KRT8 Antibody (Ascites) (Cat. #AM1827a)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KRT8 Antibody (Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | KRT8 Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRT8; CYK8; Keratin, type II cytoskeletal 8; Cytokeratin-8; Keratin-8; Type-II keratin Kb8 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 135CT23.2.2 |
| Host Species | Mouse |
| Tested Applications | WBIFIHC |
| WB:1:200~4000 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CYK8 |
|---|---|
| Alternative Names | KRT8 CYK8 Keratin type II cytoskeletal 8 Cytokeratin-8 Keratin-8 Type-II keratin Kb8 |
| Molecular Weight(MW) | 53704 Da |
| Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle |
| Tissue Specificity | Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity |
| Cellular Localization | Cytoplasm. Nucleus, nucleoplasm (By similarity). Nucleus matrix (By similarity) |
| Entrez Gene | 3856 |
|---|
Application
Western blot analysis of KRT8 Antibody (Ascites) (Cat. #AM1827a) in T47D cell line lysates (35ug/lane). KRT8 (arrow) was detected using the Mab ascites (1:1000 dilution).
Application
Confocal immunofluorescent analysis of KRT8 Antibody (Ascites)(Cat#AM1827a) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
KRT8 Antibody (Ascites) (Cat. #AM1827a)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KRT8 Antibody (Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:200~4000 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
