Application
Figure 1: Western blot analysis using anti-LIN28 mAb against NTERA-2 cell lysate (1).Application
Figure 2: Confocal immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.Application
Figure 3: Confocal immunofluorescence analysis of NTERA-2 cells using anti-LIN28 mAb (green). Blue: DRAQ5 fluorescent DNA dye.| Product Name | LIN28 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CSDD1; LIN-28; LIN28A; ZCCHC1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6D1F9 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CSDD1 LIN28 ZCCHC1 |
|---|---|
| Alternative Names | CSDD1 LIN-28 LIN28A ZCCHC1 |
| Molecular Weight(MW) | 22743 Da |
| Function | Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7 Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation |
| Tissue Specificity | Expressed in embryonic stem cells (ES cells), placenta and testis |
| Cellular Localization | Cytoplasm. Nucleus, nucleolus. Note=Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules |
| Entrez Gene | 79727 |
|---|---|
| Nucleotide Accession | NP_078950.1 |
Application
Figure 1: Western blot analysis using anti-LIN28 mAb against NTERA-2 cell lysate (1).
Application
Figure 2: Confocal immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.
Application
Figure 3: Confocal immunofluorescence analysis of NTERA-2 cells using anti-LIN28 mAb (green). Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli. |
