Application
Western blot analysis of LIN28 recombinant protein by anti-LIN28A Monoclonal Antibody (Cat.#AM1485a). LIN28 (arrow) was detected using the purified Mab.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28A Monoclonal Antibody (Cat.#AM1485a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Immunofluorescence analysis of LIN28A Monoclonal Antibody with paraffin-embedded human testis tissue. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.| Product Name | LIN28A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIN28A; CSDD1; LIN28; ZCCHC1; Protein lin-28 homolog A; Zinc finger CCHC domain-containing protein 1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 55CT58.12.1 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:200~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CSDD1 LIN28 ZCCHC1 |
|---|---|
| Alternative Names | LIN28A CSDD1 LIN28 ZCCHC1 Protein lin-28 homolog A Zinc finger CCHC domain-containing protein 1 |
| Molecular Weight(MW) | 22743 Da |
| Function | Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7 Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation |
| Tissue Specificity | Expressed in embryonic stem cells (ES cells), placenta and testis |
| Cellular Localization | Cytoplasm. Nucleus, nucleolus. Note=Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules |
| Entrez Gene | 79727 |
|---|
Application
Western blot analysis of LIN28 recombinant protein by anti-LIN28A Monoclonal Antibody (Cat.#AM1485a). LIN28 (arrow) was detected using the purified Mab.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28A Monoclonal Antibody (Cat.#AM1485a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Immunofluorescence analysis of LIN28A Monoclonal Antibody with paraffin-embedded human testis tissue. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.| Application Notes | WB:1:200~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
