Application
Immunocytochemistry: 14-3-3 gamma Antibody (HS23) - Detection of 14-3-3 gamma (green) in HepG2 cells using Nuclei (Blue) are counterstained with Hoechst 33258.Application
Western Blot: 14-3-3 gamma antibody (HS23) - 14-3-3 gamma detected in lysates using Lane 1: HeLa cell lysate, Lanes 2 & 3: bengamide treated lysates (8h and 24h, respectively).| Product Name | 14-3-3 gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [HS23] to 14-3-3 gamma14-3-3 proteins are present as multigene families in most organisms. Signal-induced phosphorylation has the ability to change protein function. Sometimes, however, phosphorylation is not enough to change a protein's function. 14-3-3 proteins play an important role of bringing signal transduction to completion. They regulate many cellular processes that are important in cancer biology, such as apoptosis and cell-cycle checkpoints. |
| Immunogen | This antibody was raised against an N-terminal fragment of human 14-3-3 gamma. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Clone Number | HS23 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| Western Blot 1:1,000: |
|
| Species Reactivity | HumanMouseChickenRatZebra FishBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KCIP-1 143 3 protein gamma 143 3gamma 14-3-3 gamma 1433 gamma 14 3 3gamma KCIP1 14 3 3 protein gamma 1433 1433 protein gamma subtype Protein kinase C inhibitor prote KCIP 1 Tyrosine3 monooxygenase/tryptophan5 monooxy 3 monooxygenase/tryptophan |
|---|---|
| Target | YWHAG |
| Tissue Specificity | This is specific for human 14-3-3 gamma. |
| Cellular Localization | Cytoplasmic |
Application
Immunocytochemistry: 14-3-3 gamma Antibody (HS23) - Detection of 14-3-3 gamma (green) in HepG2 cells using Nuclei (Blue) are counterstained with Hoechst 33258.
Application
Western Blot: 14-3-3 gamma antibody (HS23) - 14-3-3 gamma detected in lysates using Lane 1: HeLa cell lysate, Lanes 2 & 3: bengamide treated lysates (8h and 24h, respectively).| Application Notes | Western Blot 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
