Application
TOP: untransfected control; BOTTOM: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)Application
Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5μg/mL)Application
Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)| Product Name | 6X His tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal antibody [HIS.H8] to 6X His tagThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Immunogen | HHHHHH (6x His) synthetic peptide conjugated to KLH |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | HIS.H8 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIPWB |
| Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1000-1:3000.
For ICC/IF: Use at a dilution of 1:100-1:500.
For IP: Use at a dilution of 1:50-1:200.
Optimal dilutions should be determined experimentally by the end user.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 6 His His tag 6 His epitope tag HHHHHH tag HHHHHH epitope tag |
|---|---|
| Target | 6X His tag |
| Tissue Specificity | Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags) |
Application
TOP: untransfected control; BOTTOM: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
Application
Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5μg/mL)
Application
Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)| Application Notes | Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1000-1:3000.
For ICC/IF: Use at a dilution of 1:100-1:500.
For IP: Use at a dilution of 1:50-1:200.
Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. |
| Storage Buffer | 10mM PBS, pH 7.2, 0.05% NaN3 |
