WB
Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein,using HRP-conjugated Mouse anti His-Tag mAb at 1:20000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.| Product Name | HRP-conjugated Mouse anti His-Tag mAb |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal [SPM368] to 6X His tagThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Immunogen | Synthetic peptide. |
| Conjugation | HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:2000-1:10000 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 6 His His tag 6 His epitope tag HHHHHH tag HHHHHH epitope tag 6X His tag |
|---|
WB
Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein,using HRP-conjugated Mouse anti His-Tag mAb at 1:20000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.| Application Notes | WB:1:2000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
