| Product Name | ADAM10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ADAM10Proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-alpha, Notch and its ligand Delta are all membrane bound molecules, which are cleaved by proteases. ADAM10, a metalloprotease-disintegrin from the family of mammalian ADAMs (a disintegrin and metalloprotease), cleaves amyloid plaque protein, TNF-alpha, Notch, and its ligand Delta. ADAM10 contains the canonical HExxHxxxxxH zinc metalloproteinase motif. In bovine kidney, ADAM10 cleaves Type IV collagen. ADAM10 is efficiently inhibited by the endogenous MMP inhibitors TIMP1 and TIMP3, but not by TIMP2 and TIMP4. ADAM10 is widely expressed in tissues and can be found on the cell surface and within the cell. The genes encoding human, mouse and bovine ADAM10 are designated ADAM10, kuzbanian (KUZ), and MADM respectively and have 97% sequence identity with each other and with rat ADAM10. There is 45% identity between mouse and Drosophila. |
| Immunogen | A synthetic peptide corresponding to the cytoplasmic domain of human ADAM10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500 - 1:2,000. U s i n g J u r k a t w h o l e c e l l l y s a t e s , a n 8 5 k D a b a n d c a n b e d e t e c t e d , w h i c h m a y r e p r e s e n t t h e p r e c u r s o r ( p r o f o r m ) p r o t e i n . A 6 0 k D a f a i n t b a n d m a y b e d e t e c t e d i n s o m e c e l l l i n e s i n c l u d i n g J u r k a t , w h i c h a p p e a r s t o b e t h e p r o c e s s e d m a t u r e p r o t e i n . Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 102 HsT18717 ADAM10 kuz MADM 602192 O14672 |
|---|---|
| Target | ADAM10 |
| Tissue Specificity | This antibody may be used for the detection and localization of human ADAM10 and does not react with other ADAMs. It recognizes the cytoplasmic domain of ADAM10 and detects latent and active forms of the enzyme.The predicted mass of the full length human ADAM10 (748 amino acids) is 84.1 kDa. Due to glycosylation and the cysteine-rich regions, the protein migrates at 98 kDa on reduced SDS-PAGE, and 58-60 kDa when processed by furin. A smaller 691 amino acid sequence for ADAM10, lacking the transmembrane domain, has been reported with a predicted molecular weight of 77.6 kDa. |
| Cellular Localization | Cell membrane, Type I membrane protein. Also localized in the Golgi apparatus. |
| Positive Control | Jurkat whole cell lysates |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500 - 1:2,000. U s i n g J u r k a t w h o l e c e l l l y s a t e s , a n 8 5 k D a b a n d c a n b e d e t e c t e d , w h i c h m a y r e p r e s e n t t h e p r e c u r s o r ( p r o f o r m ) p r o t e i n . A 6 0 k D a f a i n t b a n d m a y b e d e t e c t e d i n s o m e c e l l l i n e s i n c l u d i n g J u r k a t , w h i c h a p p e a r s t o b e t h e p r o c e s s e d m a t u r e p r o t e i n . Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC. Avoid freeze-thaw cycles. DO NOT store below -22°C. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before u |
| Storage Buffer | Phosphate-buffered saline containing 0.02% sodium azide |
