Application
Immunofluorescence of ADAM10 in K562 cells with ADAM10 antibody at 10 μg/mL.Application
Western blot analysis of ADAM10 in Jurkat whole cell lysate with ADAM10 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunocytochemistry staining of K562 cells using ADAM10 antibody at 2 μg/mL.| Product Name | ADAM10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ADAM10Proinflammatory cytokine tumor necrosis factor-a (TNF-a)contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, Respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4). |
| Immunogen | Synthetic peptide - amino acids 732-748 of Human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ICC/IF: 2-10 μg/mL : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 102 HsT18717 ADAM10 kuz MADM 602192 O14672 |
|---|---|
| Target | ADAM10 |
| Cellular Localization | Cell membrane, Type I membrane protein. Also localized in the Golgi apparatus. |
Application
Immunofluorescence of ADAM10 in K562 cells with ADAM10 antibody at 10 μg/mL.
Application
Western blot analysis of ADAM10 in Jurkat whole cell lysate with ADAM10 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunocytochemistry staining of K562 cells using ADAM10 antibody at 2 μg/mL.| Positive Control | Jurkat whole cell lysate |
|---|---|
| Application Notes | ICC/IF: 2-10 μg/mL : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide |
