Application
Western blot of rat brain lysate showing specific immunolabeling of the ~15k alpha synuclein protein using alpha Synuclein antibody .IF
Immunofluorescence and quantification analysis of the colocalization of α-syn with VMAT2 (f) or VAMP2 (g) in primary neurons after different treatments (n = 5 biologically independent cultures).IF
Representative immunofluorescence staining images and colocalization analysis along the white line of α-syn with VMAT2 (m) or VAMP2 (n).| Product Name | alpha Synuclein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse polyclonal antibody to alpha SynucleinMay be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. |
| Immunogen | Full length human alpha synuclein expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ICC/IFIFWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PD1 PARK4 SNCA NACP MGC110988 PARK1 |
|---|---|
| Target | alpha Synuclein |
| Molecular Weight(MW) | 15 (note) |
| Cellular Localization | Membrane-bound in dopaminergic neurons. Also found in the nucleus. |
Application
Western blot of rat brain lysate showing specific immunolabeling of the ~15k alpha synuclein protein using alpha Synuclein antibody .
IF
Immunofluorescence and quantification analysis of the colocalization of α-syn with VMAT2 (f) or VAMP2 (g) in primary neurons after different treatments (n = 5 biologically independent cultures).
IF
Representative immunofluorescence staining images and colocalization analysis along the white line of α-syn with VMAT2 (m) or VAMP2 (n).| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | Ascites fluid + 0.01 % NaN3. |
