Application
Immunostaining of the Lewy body in substantia nigra neuron using Mab 4D6| Product Name | alpha Synuclein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4D6] to alpha SynucleinThe implication of alpha synuclein in neurodegenerative diseases started after the identification of non A beta component of amyloid (NAC peptide) in Alzheimeris disease (AD) brains. There is now a general group of synucleinopathies which implicates alpha synuclein in Lewy body syndromes, Parkinsonis disease, and Alzheimeris disease. Certain missense mutations in the alpha synuclein gene (A53T, A30P) have been linked to the familial Parkinsonis disease (PD). Alpha Synuclein is the major component of Lewy bodies and Lewy neuritis in sporadic PD, dementia with Lewy Bodies and Lewy Body variant of AD. Lewy bodies are composed of truncated and phosphorylated intermediate neurofilament proteins, alpha synuclein, ubiquitin and associated enzymes. The synuclein phosphoproteins (15-20 kD) are small highly conserved proteins in vertebrates. The synuclein family includes alpha and beta synucleins and loosely related gamma synuclein and synoretin. The expression is abundant in neurons and typically is localized at presynaptic terminals. Antibodies to alpha synuclein provide a specific method to detect Lewy bodies and associated pathological mechanism of PD and AD. |
| Immunogen | Recombinant human alpha synuclein, produced in E.coli. |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Clone Number | 4D6 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-FIHC-PWB |
| ELISA: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining. IHC-Fr: Use at a dilution of 1/100 - 1/1000. WB: Use at a dilution of 1/100 - 1/10000. Predicted molecular weight: 16 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | SNCA PD1 NACP PARK4 6622 MGC110988 P37840 127750 PARK1 alpha Synuclein |
|---|---|
| Target | alpha Synuclein |
| Cellular Localization | Cytoplasmic and Nuclear. Membrane-bound in dopaminergic neurons. |
Application
Immunostaining of the Lewy body in substantia nigra neuron using Mab 4D6| Application Notes | ELISA: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining. IHC-Fr: Use at a dilution of 1/100 - 1/1000. WB: Use at a dilution of 1/100 - 1/10000. Predicted molecular weight: 16 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: PBS |
