Application
Flow cytometric analysis of Hela cells using BCL10 mouse mAb (green) and negative control (purple).Application
Western blot analysis using anti-BCL10 mAb against NIH/3T3 (1), Hela (2), MCF-7 (3) and Jurkat (4) cell lysate.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using anti-BCL10 mAb with DAB staining.Application
Confocal immunofluorescence analysis of Hela cells using anti-BCL10 mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Product Name | BCL10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4F8E8H8,4F8E3H8] to BCL10This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human BCL10 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4F8E8H8,4F8E3H8 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | CIPER CLAP ME10 C-E10 BCL10 CARMEN |
|---|---|
| Target | BCL10 |
| Molecular Weight(MW) | 26 kDa (note) |
| Cellular Localization | Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. |
Application
Flow cytometric analysis of Hela cells using BCL10 mouse mAb (green) and negative control (purple).
Application
Western blot analysis using anti-BCL10 mAb against NIH/3T3 (1), Hela (2), MCF-7 (3) and Jurkat (4) cell lysate.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using anti-BCL10 mAb with DAB staining.
Application
Confocal immunofluorescence analysis of Hela cells using anti-BCL10 mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide. |
