Application
Amyloid beta A4 peptides were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 2 μg/mL for 1 hour at 15-22ºC and developed by ECL (exp. time: 30 sec.). Lane 1: bA4 (1-40) Lane 2: bA4 (1-42) Lane 3: bA4 (1-43)| Product Name | beta Amyloid (1-40) antibody [BDI350] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [BDI350] to beta Amyloid (1-40)The cerebral and vascular plaques associated with Alzheimer's disease (AD) are mainly composed of amyloid beta peptides (Ab). Ab is derived from cleavage of the amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. Ab [1-40], Ab [1-42], and Ab [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The cleavage takes place by gamma secretase during the last APP processing step. Ab [1-40], [1-42] and [1-43] peptides are major constituents of the plaques and tangles that occur in AD. Ab antibodies and peptides have been developed as tools for elucidating the biology of AD. |
| Immunogen | Peptide conjugated to KLH (C terminal). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | BDI350 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Unpurified |
| Gene Synonyms | AD1, APP, ABETA, CTFgamma, APPI, 351, ABPP, CVAP, beta Amyloid, PN2, 104760, P05067, AAA |
|---|---|
| Target | beta Amyloid (1-40) |
| Tissue Specificity | Interacts with the C terminus of beta Amyloid 1-40. Does not cross react with beta Amyloid 1-42. |
| Cellular Localization | Type I membrane protein. |
Application
Amyloid beta A4 peptides were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 2 μg/mL for 1 hour at 15-22ºC and developed by ECL (exp. time: 30 sec.). Lane 1: bA4 (1-40) Lane 2: bA4 (1-42) Lane 3: bA4 (1-43)| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide. Constituents: PBS, Polyethylene glycol, Sucrose |
