| Product Name | beta Amyloid antibody [3G5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3G5] to beta AmyloidThe cerebral and vascular plaques associated with Alzheimer's disease are mainly composed of Amyloid beta peptides. beta Amyloid is derived from cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides result from cleavage of Amyloid precursor protein after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last Amyloid precursor protein processing step. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides are major constituents of the plaques and tangles that occur in Alzheimer's disease. beta Amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimer's disease. |
| Immunogen | A 28 amino acid synthetic peptide (human) derived from the b-amyloid peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3G5 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| For ELISA: Use at a concentration of 0.5-1.0 μg/ml. For IHC: Use at an assay dependent dilution. For WB: Use at a concentration of 0.1-0.2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | AD1, APP, ABETA, CTFgamma, APPI, 351, ABPP, CVAP, beta Amyloid, PN2, 104760, P05067, AAA |
|---|---|
| Target | beta Amyloid |
| Tissue Specificity | Reacts with the 4-5kD b-amyloid peptide (b-AP). High concentrations of antibody will also react with A-a, B-a and g subunits of fibrinogen. |
| Cellular Localization | Type I membrane protein |
| Light Chain Type | kappa |
| Application Notes | For ELISA: Use at a concentration of 0.5-1.0 μg/ml. For IHC: Use at an assay dependent dilution. For WB: Use at a concentration of 0.1-0.2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.05% sodium azide |
