Application
Whole cell extract of Chicken fibroblasts was separated on SDS-PAGE, and blotted with monoclonal Anti-ß Tubulin . The antibody was developed with Goat APA-Mouse Fab, Peroxidase conjugate and AEC substrate. Lanes A: Antibody dilution 1:200 B: Negative control (only secondary antibody)Application
Double immunofluorescent labeling PTK-1 cells in anaphase and interphase stained with Monoclonal and Anti-β-Tubulin (clone no. 2-28-33, and Goat Anti-Mouse IgG-FITC, F(ab′)2 fragment and anti-centromere from human sera with Goat Anti-Human IgG-Cy3. Chromosomes are counterstained with Hoechst. Images were acquired on a videomicroscope with a cooled CCD camera.| Product Name | beta Tubulin antibody [2-28-33] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2 - 28 - 33] to beta TubulinTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. |
| Immunogen | Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2-28-33 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC: Use at an assay dependent dilution. IF: Use at a dilution of 1/1000, this dilution was determined by indirect immunofluorescent labeling of cultured human fibroblasts. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Mammalian speciesSea Urchin |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P07437, OK/SWcl.56, M40, MGC16435, TUBB5, 191130, TUBB, beta Tubulin, OK/SW-cl.56, MGC117247, TUBB1, 203068, OK/SW cl.56 |
|---|---|
| Target | beta Tubulin |
| Tissue Specificity | recognizes the two major beta tubulin isotypes and one of the minor beta-tubulin isotypes of the free-living soil nematode Caenorhabditis elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes, to neuronal axons of the larva of Ciona intestinalis (ascidian), and to tubulin of chicken and mammals (e.g., cultured human fibroblasts, bovine, and rat brain tissue). In C. elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and beta-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels, and found reactive with heat-denatured and reduced mammalian preparations in immunoblotting. |
| Cellular Localization | Cytoplasmic |
Application
Whole cell extract of Chicken fibroblasts was separated on SDS-PAGE, and blotted with monoclonal Anti-ß Tubulin . The antibody was developed with Goat APA-Mouse Fab, Peroxidase conjugate and AEC substrate. Lanes A: Antibody dilution 1:200 B: Negative control (only secondary antibody)
Application
Double immunofluorescent labeling PTK-1 cells in anaphase and interphase stained with Monoclonal and Anti-β-Tubulin (clone no. 2-28-33, and Goat Anti-Mouse IgG-FITC, F(ab′)2 fragment and anti-centromere from human sera with Goat Anti-Human IgG-Cy3. Chromosomes are counterstained with Hoechst. Images were acquired on a videomicroscope with a cooled CCD camera.| Positive Control | Cultured human fibroblasts |
|---|---|
| Application Notes | ICC: Use at an assay dependent dilution. IF: Use at a dilution of 1/1000, this dilution was determined by indirect immunofluorescent labeling of cultured human fibroblasts. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at −20°C. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Raw Ascites |
