| Product Name | beta Tubulin antibody [DM1B] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [DM1B] to beta TubulinCytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies. |
| Immunogen | Native chicken brain microtubules |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DM1B |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For IF: Use at a concentration of 4 - 6 μg/ m l. For WB: Use at a concentration of 4 -6μg/ m l. Detects a band of approximately 60 kDa. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRatGerbil |
| Concentration | 0.2mg/ml |
| Purification | Unpurified |
| Gene Synonyms | P07437, OK/SWcl.56, M40, MGC16435, TUBB5, 191130, TUBB, beta Tubulin, OK/SW-cl.56, MGC117247, TUBB1, 203068, OK/SW cl.56 |
|---|---|
| Target | beta Tubulin |
| Light Chain Type | kappa |
| Positive Control | Any cell line |
|---|---|
| Application Notes | For IF: Use at a concentration of 4 - 6 μg/ m l. For WB: Use at a concentration of 4 -6μg/ m l. Detects a band of approximately 60 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 50 mM sodium phosphate buffer containing 0.2% gelatin and =0.1% sodium azide |
