Application
Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-beta-tubulin (TU-06) (detection by Goat anti-mouse IgM Cy®5). Nucleus is stained with DAPI (blue).Application
Western Blotting analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line. Lane 1: negative control. Lane 2,3,4,5,6: immunostaining with anti-beta-tubulin (TU-06 ; dilution 0,5 μg/ml, 1 μg/ml, 2 μg/ml, 4 μg/ml, 5 μg/ml)| Product Name | beta Tubulin antibody [TU-06] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [TU - 06] to beta TubulinTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. |
| Immunogen | Beta-subunits of tubulin (purified) (Pig). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | TU-06 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PIPWB |
| ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMousePigRatCarpHamsterBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Unpurified |
| Gene Synonyms | P07437, OK/SWcl.56, M40, MGC16435, TUBB5, 191130, TUBB, beta Tubulin, OK/SW-cl.56, MGC117247, TUBB1, 203068, OK/SW cl.56 |
|---|---|
| Target | beta Tubulin |
| Tissue Specificity | N-terminal structural domain of b-tubulin of wide variety of species. TU-06 reacts with all charge variants of tubulin. |
| Cellular Localization | Cytoplasmic |
Application
Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-beta-tubulin (TU-06) (detection by Goat anti-mouse IgM Cy®5). Nucleus is stained with DAPI (blue).
Application
Western Blotting analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line. Lane 1: negative control. Lane 2,3,4,5,6: immunostaining with anti-beta-tubulin (TU-06 ; dilution 0,5 μg/ml, 1 μg/ml, 2 μg/ml, 4 μg/ml, 5 μg/ml)| Application Notes | ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
