Application
WB analysis of (1) HeLa, (2) U87, (3) P19, (4) Neuro-2a, (5) PC-12, (6) COS-7, (7) CHO, (8) MDBK and (9) MDCK lysates using beta Tubulin antibody [D66] at a concentration of 1 μg/ml.Application
Total cell extract of PC-12 was separated on SDS-PAGE and blotted with Monoclonal Anti-β-tubulin . The antibody was developed with Goat Anti-Mouse IgG, alkaline phosphatase conjugate and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibodyApplication
ICC/IF analysis of SH-SY-5Y cells using beta Tubulin antibody [D66] at a concentration of 0.5 μg/ml (red) and DAPI (blue).| Product Name | beta Tubulin antibody [D66] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [D66] to beta TubulinTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. |
| Immunogen | The immunogen corresponding to a region within amino acids 427 and 432 of sea urchin β2-tubulin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | D66 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIPWB |
| ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution (3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 1 μg/ml. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseDogRatChlamydomonas ReinhardtiiHamsterRabbitSea UrchinBovine |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | P07437, OK/SWcl.56, M40, MGC16435, TUBB5, 191130, TUBB, beta Tubulin, OK/SW-cl.56, MGC117247, TUBB1, 203068, OK/SW cl.56 |
|---|---|
| Target | beta Tubulin |
| Tissue Specificity | Recognizes all mouse brain beta tubulin isoforms (beta and beta subpopulations), and sea urchin beta 2 tubulin. |
| Cellular Localization | Cytoplasmic |
Application
WB analysis of (1) HeLa, (2) U87, (3) P19, (4) Neuro-2a, (5) PC-12, (6) COS-7, (7) CHO, (8) MDBK and (9) MDCK lysates using beta Tubulin antibody [D66] at a concentration of 1 μg/ml.
Application
Total cell extract of PC-12 was separated on SDS-PAGE and blotted with Monoclonal Anti-β-tubulin . The antibody was developed with Goat Anti-Mouse IgG, alkaline phosphatase conjugate and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody
Application
ICC/IF analysis of SH-SY-5Y cells using beta Tubulin antibody [D66] at a concentration of 0.5 μg/ml (red) and DAPI (blue).| Positive Control | Cultured rat adrenal pheochromocytoma (PC-12) cells |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution (3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 1 μg/ml. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.01M PBS. pH 7.4 containing 1% BSA and 15mM sodium azide |
