Application
C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C9orf72 protein stained by C9orf72 antibody [GT779] diluted at 1:250. Blue: Hoechst 33342 staining.Application
Immunoprecipitation of C9orf72 protein from SK-N-SH whole cell extracts using 5 μg of C9orf72 antibody [GT779] . Western blot analysis was performed using C9orf72 antibody [GT779] . EasyBlot anti-Mouse IgG was used as a secondary reagent.Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. C9orf72 antibody [GT779] diluted at 1:500.Application
C9orf72 antibody detects C9orf72 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | C9orf72 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT779] to C9orf72 (chromosome 9 open reading frame 72) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT779 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevisXenopus laevis (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ALSFTD FTDALS chromosome 9 open reading frame 72 |
|---|---|
| Target | C9orf72 |
| Molecular Weight(MW) | 54 kDa (note) (4) |
| Tissue Specificity | Knockdown/Knockout validation was supported by customer review data |
Application
C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C9orf72 protein stained by C9orf72 antibody [GT779] diluted at 1:250. Blue: Hoechst 33342 staining.
Application
Immunoprecipitation of C9orf72 protein from SK-N-SH whole cell extracts using 5 μg of C9orf72 antibody [GT779] . Western blot analysis was performed using C9orf72 antibody [GT779] . EasyBlot anti-Mouse IgG was used as a secondary reagent.
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. C9orf72 antibody [GT779] diluted at 1:500.
Application
C9orf72 antibody detects C9orf72 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
