| Product Name | Caspase 4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Caspase 4Caspase 4 is a 377 amino acids protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an upstream caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67% homology to mature caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis. |
| Immunogen | Synthetic peptide: KQISPNKKAHPNMEAGPPE conjugated to KLH, corresponding to amino acids 81-98 of Human Caspase 4 (with N-terminally added K). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| For IP: Use at a concentration of 5-10 μg using 300 μg lysate of human ECV304 endothelial cells. For WB(chemiluminescent): Use at a dilution of 1:250 using whole cell extract of human ECV304 endotheloid cells. Optimal dilutions/concentrations should be determined by the researcher.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 602664 ICERELII ICE(rel)II 837 P49662 CASP4 Caspase4 Caspase 4 TX ICH-2 Mih1/TX ICH2 ICEREL-II ICH 2 ICEREL II |
|---|---|
| Target | Caspase 4 |
| Application Notes | For IP: Use at a concentration of 5-10 μg using 300 μg lysate of human ECV304 endothelial cells. For WB(chemiluminescent): Use at a dilution of 1:250 using whole cell extract of human ECV304 endotheloid cells. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide |
